ONLINE DRIVERS MEDIA

Dont forget that cyclists are not the only group of athletes who can benefit from bicarbonate supplementation. Strength trainees who spend hours in the gym and train at high intensities will also benefit!

I know that most of you are into resistance not endurance training. So, before I even get into the discussion of the experimental procedures and the results of the latest study from the Institute of Sports and Preventive Medicine at the Saarland University in Saarbrücken, Germany, I would like to point you to an older SuppVersity article which indicates that bicarbonate supplementation is able to Up Your Squat (+27%) & Bench Press (+6%) Within 60 Min" (read more).

Now that youve hopefully put away your prejudices against "that endurance supplement", lets get to the previously mentioned study by Florian Egger, Tim Meyer, Ulf Such, and Anne Hecksteden (thanks to Conrad P. Earnest for bringing this to my attention).
To investigate the effects of BICA supplementation on performance during prolonged, high-intensity cycling to exhaustion in well-trained athletes, the scientists from the Saarland University recruited 6 male and 5 female "well-trained" cyclists (mean ± SD: age 24±8 y, BMI 21.3±1.7, VO2peak 67.3±9.8 ml/kg/min - the VO2peak value tells you that they were fit ;-).

In a double-blind, randomized cross-over design, the subjects underwent two stepwise incremental exercise tests and two constant load tests (with two phases) on an electrically braked cycle ergometer (Excalibur Sport, Lode, Groningen, The Netherlands).

Figure 1: Schematic representation of the general design.Time interval between tests is specified in days (d). Data are presented as means ± standard deviation respectively, with minimum (min) and maximum (max) values (Egger. 2014).

As the overview of the study design in Figure 1 tells you, each test type was completed twice. Once after the ingestion of 0.3 g/kg sodium bicarbonate (yes, thats roughly 24g for someone who weighs 80g and should not be consumed too fast, because otherwise it may trigger diarrhea) or a placebo supplement in form of 4 g sodium chloride that was chosen to make sure that any benefits that were observed were due to the natrium, not the bicarbonate content of sodium bicarbonate.
Both the plain salt and the sodium bicarbonate were solved in 0.7 l water. The outcome measures were simple: Only if the subjects were able to pedal significantly longer until they were exhausted in the standardized constant load test, sodium bicarbonate could be considered to have practically relevant performance enhancing effects (maximum performance in the stepwise incremental exercise test, i.e. maximal workload and VO2peak were used as secondary outcomes).

Figure 2: Blood lactate (BLa) concentrations after ingestion (post drink) and during constant load tests (mean ± SD) for the BICA and placebo trials (Egger. 2014)

The other parameters the scientists measured, i.e. the blood lactate [BLa], pH, and bicarbonate concentration, were merely used determine the mechanisms for the potential improvements in exercise performance.

Speaking of auxiliary measures, if you take a look at Figure 2 you will see that the blood pH dropped significantly right after the ingestion of the bicarbonate supplement and remained "low" throughout the trial and afterwards. An observation that does not come unexpected. Previous trials have after all shown that its the ability of bicarbonate to blunt the high-intensity exercise related perturbations in both blood and muscle acid-base that keeps the maximal work rate up and leads to performance increases compared to placebo supplements.
These performance decrements are caused by the accumulation of hydrogen ions (H+) in the myoplasm and their detrimental effects on myofilament interaction, glycolytic flux and sarcoplasmatic reticulum function. As Egger et al. point out

"[t]he ability of the body to prevent or delay these force limiting processes is determined by the capacity of its intrinsic buffering systems, which counteract the accumulation of H+ both inside and outside the cell," (Egger. 2014)

which explains why the benefits of both beta alanine (which increases the intra-cellular buffering capacity) and bicarbonate are most pronounced in athletes competing in high intensity sports.

Figure 3: Time to exhaustion and maximal workload (total) and maximal workload at the individual anaerobic threshold (IAT) during the bicarbonate and placebo trials (Egger. 2014).

Apropos ergogenic effects: I already gave it away in the headline. The consumption of the bicarbonate supplement lead to immediate increases in the time to exhaustion with 49.5 ±11.5 min being the maximum in the bicarbonate and 45.0±9.5 min being the maximum in the placebo condition.

The maximal workload in the stepwise incremental tests (BICA: 341±66 W; placebo: 339±67 W) and workload at IAT (BICA: 234±5.5 W; placebo 233±5.7 W), on the other hand, did not differ significantly.
References:

• Egger F, Meyer T, Such U, Hecksteden A. "Effects of Sodium Bicarbonate on High-Intensity Endurance Performance in Cyclists: A Double-Blind, Randomized Cross-Over Trial". PLoS ONE 9.12 (2014): e114729.
• Hobson, Ruth M., et al. "Effects of ?-alanine supplementation on exercise performance: a meta-analysis." Amino acids 43.1 (2012): 25-37.

Its obviously to have the 24h effects on testosterone and cortisol than only those measured after the workout , but can we make solid conclusions based on the additional data?

In spite of the fact that the acute testosterone and cortisol response to exercise appears to have little direct effects on the overall training outcome (Schoenfeld. 2013), acute increase in cortisol and reductions in testosterone, i.e. a decrease in the testosterone:cortisol ratio is a classic feature of overtraining and can very well blunt, if not reverse the beneficial effects of exercise on your health and body composition.

Against that background a recent experiment that was conducted by researchers from the University of Chieti-Pescara in Italy could be of great interest to everyone who is performing high intensity interval training on a regular basis. Why?

Well, in contrast to previous studies, Blasio et al. investigated both the acute and 24h effects of a high intensity interval resistance training regimen in trained young men.
To characterize the effects on heart rate and hormonal responses the subjects, eight trained, healthy trained men (28.61 ±3.51 yrs), performed three different workouts which had the same exercises, the same load and number of repetitions for each exercise, but different exercise order, recovery and speed of execution.

• RANDOM workout: the assigned goal was to complete the assigned repetitions respecting only two duties. The first one was don’t stop until all of the repetitions were completed; the second was that there were no assigned order of execution of exercises and no assigned consecutive repetitions to complete.
  
  Participants were thus free to choose both the order of exercises and number of consecutive repetitions for each exercise (i.e. 2 repetitions of kettlebell swing, 10 repetitions of medicine ball slam, 20 repetitions of squat, 4 repetitions of spin with Bulgarian bag, etc.).
  
  No recovery period was assigned, except the time necessary to move from a station to another, and no speed of execution of exercises was assigned: participants were free to choose the preferred speed. 
• LADDER workout: respecting the following order of execution, kettlebell swing, medicine ball slam, spin with Bulgarian bag, squat, pull-up, burpee, participants had to complete the total repetitions according to a pyramidal scheme (e.g. 1st lap 10 repetitions at each exercise, 2nd lap 9 repetitions at each exercise) until the total number of repetitions of each exercise was executed.
  
  Each lap of the circuit was followed by 1 minute of recovery. No speed of execution of exercises was assigned: participants were free to choose the preferred speed. 
• AS SOON AS POSSIBLE (ASAP) workout : respecting the following order of execution, kettlebel swing, medicine ball slam, spin with Bulgarian bag, squat, pull-up, burpee, participants had to complete the total volume in six laps executed as soon as possible.
  
  During each lap participants had to complete the sixth part of total number of repetitions of each exercise without rest among exercises. Each lap of the circuit was followed by 1 minute of recovery.

Salivary samples were collected before and after each workout, at 11:00 p.m. and at 7:00 a.m. of the following day. Salive was also collected during a non-training day. Similarly, before and after the workout, plasma lactate was measured while a beat-to-beat heart rate recording was executed during each workout. Cortisol (C) and testosterone (T) were measured in salivary samples.

2h before the workouts the subjects who had to abstain from sexual intercourse, stimulants and alcohol from 2 days before to the experimental days and until 9:00 a.m. of the following day, consumed a standardized meal that was lower to 400 and consisted of 33 cl of water, 35 cl of orange juice and two 30 g energy bars (Power Sport Double Use, Enervit, Milan, Italy).

Lets look at the results

While the protocols elicited the same heart rate response (the major part of each workout was spent between 80 and 100% of maximal heart rate, confirming the high cardiovascular intensity of the workouts), they elicited different hormonal and lactate variations with the LADDER workout producing the lowest lactate increase and the RANDOM workout eliciting the highest lactate, cortisol and testosterone increases.

Figure 1: Relative changes in hormone and lactate concentration in response to the workouts (Di Blasio. 2014)

When C was considered in ratio with T no significant differences have been shown among workouts-induced variations. Results of the analysis of covariance, executed on significantly modified variables, confirmed that basal hormonal and lactate values did not influence their variations.

When they studied the effects of workouts on prolonged hormones production (i.e. until the morning following the morning, di Blasio et. al. found that observed that observed that

"C had both time (F=179.723; p < 0.001) and group × time effect (F=10.942; p < 0.001): while during non-training day there is a physiological decline of C production at 11:00 p.m., during training days its decline is not present but seems to have a continuous increase from 7:00 p.m. to 7:00 a.m." (Di Blasio. 2014)

For the testosterone production the authors found both time (F=443.340; p < 0.001) and group × time effect (F=3.254; p=0.008) even if the group × time effect seems determined by the samples collected at 7:00 p.m., so that the effects cannot be ascribed fully / exclusively to the workout.

Figure 2: 23h hormone profile after the RANDOM, LADDER, ASAP workouts on a control day (di Blasio. 2014)

What is most interesting, though, is the cortisol to testosterone ratio. It shows the greatest inter-group differences and could potentially be of great physiological relevance (Crowley. 1996). In that, the LADDER workout has the most negative effect, as it will totally blunt the natural decline of the C:T ratio at noon.
References:

• Crowley, Michael A., and Kathleen S. Matt. "Hormonal regulation of skeletal muscle hypertrophy in rats: the testosterone to cortisol ratio." European journal of applied physiology and occupational physiology 73.1-2 (1996): 66-72. 
• Schoenfeld, Brad J. "Postexercise hypertrophic adaptations: a reexamination of the hormone hypothesis and its applicability to resistance training program design." The Journal of Strength & Conditioning Research 27.6 (2013): 1720-1730.

The results of the study and hand strongly suggest, but dont finally prove that a brief leg-focused HIIT like spinning will not impair your biceps gains even if its done immediately before weight training.

You will probably have read about the problem that doing endurance before or immediately after resistance training will blunt the protein synthetic response to exercise. In fact, I have written about this topic repeatedly. The latest article was published only two weeks ago ("How Much Cardio Messes W/ Your Weight Training Results Depends on Recovery Times: Cardio + Weights in One Session vs. AM + PM Training vs. Doing Each on One Day" | read more).

Now, a recent study from the Swedish School of Sport and Health Sciences shows that the question whether "cardio" in the broadest sense will interfere with strength training gains cannot be answered with a simple yes or no.
In their study, Moberg, et al. (2014)set out to conduct a series of studies to examine the influence of endurance exercise on the acute stimulatory effect of resistance exercise on anabolic processes. To this ends, the effect of endurance exercise on a previously inactive muscle was investigated. The aim was to examine the influence of resistance exercise on mTORC1-signaling and rate of protein synthesis in the triceps brachii muscle with or without preceding intervals of high-intensity cycling.

Eight trained males performed, in a randomized fashion, two sessions of heavy resistance exercise (RE) with the triceps muscles, where one session was preceded by intervals of high-intensity cycling (E+RE), 5 x 4 min at 85% of VO2 peak. Mixed muscle protein fractional synthetic rate (FSR) was measured at rest, prior to exercise, and during a 3 hour recovery period following exercise by continuous infusion of L-[ring-13C6] phenylalanine. Muscle biopsies from the triceps brachii was collected twice at rest separated by three hours, directly after resistance exercise and following 90 and 180 min of recovery. Signalling in the mTORC1-and AMPK-pathway was assessed using western blot technique.
It is important to point out that the same amount of work with regard to load, total number of repetitions and total time under tension was performed in the two trials.

Figure 1: Fractional muscle protein synthesis response in the biceps brachii and corresponding changes in signalling proteins in the HIIT + resistance training (E+RE) and the resistance training only trial (Moberg. 2014).

Unlike in previous studies, where the "cardio" exercise was usually a steady state exercise and the muscle protein synthesis was measured in muscles that were trained during the "cardio" and resistance training session, mostly specifically the leg muscles, the muscle protein fractional synthesis rates (FSR) in the study at hand increased from 0.050 ± 0.006 %/h at rest to 0.078 ± 0.008 and 0.082 ± 0.0016 %/h following E+RE and RE, respectively, with no difference between trials.

The same goes for the signalling protein AMPK and eEF2,  as well as for the alleged protein synthesis gauge mTOR which was increased by 76% and 108% above rest directly after the E+RE and RE, respectively, and remained elevated in both trials during the entire recovery period.
References:

• Lundberg, Tommy R., et al. "Aerobic exercise does not compromise muscle hypertrophy response to short-term resistance training." Journal of Applied Physiology 114.1 (2013): 81-89.
• McCarthy, J., et al. "Combined strength and endurance training: Functional and morphological adaptations to ten weeks of training." No. NHRC-92-26. Naval Health Research Center San Diego CA, 1992.
• Moberg, Marcus, et al. "High-intensity cycling performed prior to resistance exercise does not influence mTORC1-signaling and the rate of muscle protein synthesis in the triceps brachii." 19th annual Congress of the European College of Sport Science, 2-5 July 2014, Amsterdam The Netherlands. 2014.